Dividing participants into four groups, from lowest fried food intake to highest, they found no significant difference in heart disease.
There were 606 incidents linked to heart disease in total, but they were split relatively evenly between the four groups.
The authors concluded: "In a Mediterranean country where olive and sunflower oils are the most commonly used fats for frying, and where large amounts of fried foods are consumed both at and away from home, no association was observed between fried food consumption and the risk of coronary heart disease or death."
Commenting on the findings in the BMJ, Professor Michael Leitzmann of the University of Regensburg in Germany said two other studies - one from Costa Rica and another by an international team - had also failed to find strong evidence of a link.
He said: "Taken together, the myth that frying food is generally bad for the heart is not supported by available evidence.
"However, this does not mean that frequent meals of fish and chips will have no health consequences."
Fried food did contain more calories, he said, while it had also been linked to high blood pressure and obesity.
The authors of the Spanish study noted that the findings could only really be extrapolated to other Mediterranean countries with similar diets, whose people tended to fry 'fresh' with olive and sunflower oil.
Fried foods from modern American-style takeaways were different, they argued, because these tended to have been cooked in re-used oils, higher in transfats.
In addition, such takeaways tended to contain much more salt, known to increase blood pressure and heart disease risk.
However, more and more people in Britain are now frying with olive oil or sunflower oil. Britain now consumes around 28 million litres of olive oil a year double that sold a decade ago.
Half British households now use it regularly in some way, although not necessarily for frying, compared to a third 10 years ago.
Victoria Taylor, senior heart health dietitian at the British Heart Foundation, said: "Before we all reach for the frying pan it's important to remember that this was a study of a Mediterranean diet, rather than British fish and chips.
"Our diet in the UK will differ from Spain, so we cannot say that this result would be the same for us too.
"Participants in this study used unsaturated fats such as olive and sunflower oil to fry their food.
"We currently recommend swapping saturated fats like butter, lard or palm oil for unsaturated fats as a way of keeping your cholesterol down and this study gives further cause to make that switch.
"Regardless of the cooking methods used, consuming foods with high fat content means a high calorie intake. This can lead to weight gain and obesity, which is a risk factor for heart disease.
"A well-balanced diet, with plenty of fruit and veg and only a small amount of high fat foods, is best for a healthy heart."

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